After DNA shuffling, the library was submitted to screening using the modified Dpl-WS assay.
In each case a library was created and 288 clones were screened using the modified Dpl-WS assay.
Likewise, functional screening using the modified Dpl-WS assay indicated that 0.6% of screened clones presented activities that were significantly higher (>5CV) than the mean value of the activity of Tx-Xyn-AF7 clones.
Three white spotted individuals in Pedigree 1 (04-053, 04-053, 04-054) exhibited "null" alleles for a W genotyping assay, demonstrating neither the presence of the W allele nor the wild-type (w) allele.
The University of Bristol Transcriptomics Facility carried out the analysis on Affymetrix GeneChip® Human Gene 1.0 ST Arrays, using the Affymetrix whole transcript (W T) assay.
Growth was assayed on selective plates lacking tryptophan and leucine (–W, –L: transformation control) and interaction was scored on plates lacking histidine, adenine, leucine and tryptophan (–H, –A, –L, –W: interaction assay).
Secreted APPβ was analysed using the sAPPβ-w (highly sensitive) Assay Kit IBL (code 27732, Immuno-Biological Laboratories, HamburGermanyaccordingrding to the manufacturer description.
SiFs-deposited glass slides were coated with self-adhesive silicon isolators, containing oval wells (2.0 mm D×32 mm L×19 mm W) prior to the assay fabrication and subsequent fluorescence experiments.
(P – R ) Wing size genetic interaction assay.
The wild-type stock used for Western blot assay was w.
The membranes were washed with 200 μL of buffer W in the MultiScreen Assay System assembly.
