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One wrong fold can throw the whole building out of alignment.
While this segment is good for modeling the structure locally, extension of the alignment to cover the entire domain results in a wrong fold prediction.
Hits from the same SCOP fold as that of known structure was considered as correct fold assignments and hits belonging to different SCOP folds were regarded as wrong fold assignment.
For estimating lDDT scores of random protein pairs, 200 protein models with wrong fold were generated by selecting pairs of structures with different CATH topologies, generating models by rebuilding side chains on the backbone of the other protein, and computing lDDT scores for these decoy models.
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We speculate that the fusion protein TrxA expressed in the pET32a vector could have increased the load of the cells, resulting in the wrong folding of the MCO, which may become an inclusion body.
One can see, how these indices increase over time and the HSOM approaches a stable state which seems to show no drastic topologic distortions such as wrong folds.
Chaperones play a key role in the heat shock response, as they are involved in promoting the acquisition of the native conformation by proteins that suffered denaturation and present the wrong folding.
If you misfeed some paper - insert it at the wrong angle, or folded over - the Fellowes will either step on the turbo boost and power through it, or reverse gears to expel the misfed sheet.
I don't know how long to dwell on this: the fold-out bed is not the first we have seen on this column, and it won't be the last, and there's not something tangibly wrong with a fold-out bed – a bed's a bed, even if it's a mildly inconvenient one!
With respect to wrong assignments of folds, very low error rates (below 1%%) were observed for all the five methods.
This was due to wrong positioning with folding of the CNB, in one case; in the other case a mucinous carcinoma was found in a separate section of the CNB, which was examined in paraffin section slides only.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com