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Titration of a compound that blocks cell migration (the actin inhibitor cytochalasin D) shows complete inhibition of migration at 1 μM, as seen by a sharply delineated wound edge and a wider wound width [Figure 2A].
The gross observational data collected on wound healing (degree of inflammation, edema, erythema, induration, amount of exudate, wound-bed environment, and re-injury/secondary wounds) (data not shown) supported the significant improvements in wound width, re-epithelialization, granulation tissue formation, and wound maturity seen with topical application of SCIO-469 compared to vehicle alone.
Microscopic pictures were taken every 6 hr for accurate measurement of wound width.
Two parallel lines were drawn at the scratch boundaries by a single masked observer to demarcate wound width.
The wound width was photographed under a light microscope.
Cells were scratched and the wound width was photographed.
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Percent relative wound widths were calculated, and the statistical significance in the case of relative wound widths were calculated using SPSS package.
Photographic representation of wound widths of each formulation studied is given in Figure 4b.
Open image in new window Figure 4 Percent relative wound widths and photo samples of 5-FU-loaded CP and GMS SLNs.
The wound widths were measured using the laser capture microdissection microscope using PalmRobo software after 24 and 48 h of incubation.
(a) Percent relative wound widths of 5-FU-loaded CP and GMS SLNs compared to 5-FU solution at 48 h; (b) photo samples of wound widths of 5-FU-loaded CP and GMS SLNs, 5-FU solution as well as their respective blank nanoparticles taken using the laser capture microdissection microscope.
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