The exit wound, lined with strips of lung and heart and blasted rib bone, was three inches across.
4 days after radiation, a wound line was created in each dish.
A sterile 200 µl pipette tip was used to create three separate parallel wounds and migration of the cells across the wound line was assessed after 24 hours.
A straight wound line was made in the monolayer of the cells.
Pictures of cells along the wound line were taken by phase-contrast microscope.
Furthermore, IPF fibroblasts but not NHLFs induced a transient increase in mesenchymal marker expression in the wound lining epithelial cells.
Cells invading the wound line were observed under an inverted phase-contrast microscope using 20×, Leica DMR, Germany.
Filaments were applied from underneath the chamber through openings in the mesh floor to an area 5 mm adjacent to the abdominal wound line (ACT) or to an area in the plantar surface of the left foot (PWT).
She also delivered a wounding line telling him that there were "undocumented immigrants in America who are paying more federal income tax than a billionaire".
