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4 days after radiation, a wound line was created in each dish.
A sterile 200 µl pipette tip was used to create three separate parallel wounds and migration of the cells across the wound line was assessed after 24 hours.
Pictures of cells along the wound line were taken by phase-contrast microscope.
A straight wound line was made in the monolayer of the cells.
Cells invading the wound line were observed under an inverted phase-contrast microscope using 20×, Leica DMR, Germany.
Filaments were applied from underneath the chamber through openings in the mesh floor to an area 5 mm adjacent to the abdominal wound line (ACT) or to an area in the plantar surface of the left foot (PWT).
Similar(50)
The exit wound, lined with strips of lung and heart and blasted rib bone, was three inches across.
Scratch wound lines were made with a fine sterile needle.
Furthermore, IPF fibroblasts but not NHLFs induced a transient increase in mesenchymal marker expression in the wound lining epithelial cells.
Moreover, the migration distances between the wound lines in inhibitor-treated cells were longer than control cells after 24 hours of culture.
She also delivered a wounding line telling him that there were "undocumented immigrants in America who are paying more federal income tax than a billionaire".
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com