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The values are the means of three independent wound fields from three independent experiments (n = 9).
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The latter likely accumulated in the wound field during the initial clotting upon surgery.
The previous finding that lack of fibrin ogen) in the wound field rescues the requirement for Plg to achieve timely healing [18] further corroborates that the primary role for Plg in wound healing is fibrinolysis.
While stem cells from the hair follicles (HF) can contribute to the healing process by migrating into the wound field to help reestablishing the epithelial barrier [9][10][11], an emerging body of evidence suggests that the interfollicular (IF) stem cells and their derived transient amplifying cells play a primary role in epidermal homeostasis and wound repair [12],[12]10][12].
In wound field of positive control (5% FCS) were 21.4 ± 6.4% cells.
Representative images focused on the center of the wound field were photographed.
The cell monolayer was scraped with a pipette tip to create an artificial wound field.
After serum starvation for 24 h, the inserts were removed to create a wound field.
Migration into the wound field was determined by using manual fixing with cell stain solution according to manufacturer's instructions.
After serum starvation for 24 h, inserts were removed with sterile forceps to create a wound field of ∼500 μm.
All the images were scored using an ordinal 0 4 scoring system of wound vascularization, with each wound field divided into 12 clock hours.
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