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Rigorous exclusion of piwi as relevant for Wnt-mediated germ cell differentiation regulation would require an experiment in which piwi is expressed in ISCs lacking arm or dsh and assessing its ability to rescue the defects observed.
It is likely that a proportion of the cDNAs considered non-significant in this study are really false negatives due to the threshold P-value used however, the detection of accurate patterns for these cDNAs would require an experiment with greater statistical power.
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For example the phrase "occupy the MP-bound sites" sounds like there is evidence that simultaneous binding was demonstrated; however, this would require a reChIP experiment, and such work was not done.
The reviewer understands that doing ribosome profiling this way would require a new experiment and he does not expect the authors doing it for this work, but the issue needs to be at least caveated.
For comparison, the same change in hydrogen content from pressurized gas loading experiments would require an increase in hydrogen fugacity from about 6 to 1420 atm.
This study could be potentially strengthened by in vivo experiments but would require an extremely large cohort of animals so was deemed as not justified.
But to count the boron isotopes precisely would require a much longer experiment — days instead of hours — and that runs into logistical problem of conducting science on famous museum artifacts.
Such an experiment would require a mechanism to change the internal representation of the subject (as one state lasting for ten seconds or as ten states lasting for one second each).
A more accurate analysis would require a series of dedicated experiments providing estimates of the composition of these tissues and assessing the stability of this composition over time.
Then, in January, one of her eight researchers wanted to study how white blood cells respond to early infection, an experiment that would require a virulent strain.
I have several suggestions that I think would help, including an experiment that would require a change in either state or federal regulations: giving some high-quality health care providers the opportunity to practice in a world without malpractice lawsuits.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com