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We sought to make neutral thiazolium replacements that would be substrates for TPPK, while not necessarily needing thiamine transporters (ThTr1 and ThTr2) for cell penetration.
This difference could reflect the fact that these SSBs would be substrates for a SSB repair (SSBR) pathway that would recruit proteins different from those at the SSB generated during BER (Caldecott, 2008).
Interestingly, only CA25 and several of the chimaeras had positive scores, leading to the prediction that these proteins, but not Y21 and UP30, would be substrates for the S. cerevisiae GPI-T.
This difference could reflect the fact that these SSBs would be substrates for a SSB repair (SSBR) pathway that would recruit proteins different from those at the SSB generated during BER (Hanawalt and Spivak, 2008)." (The rest of the issues raised in this point are similar to those raised in point 4, which we addressed above).
If there is misalignment of repeat units during recombinational repair (shown to be required for tract alterations) (Kelly et al. 2011), there will be potential C/C or G/G mispairs at nucleotides 14 and 22 in each repeat; these would be substrates for mismatch repair that could also affect tract length during the repair process.
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On the basis of the above, we predicted that peptides based on the processing sites would be good substrates for rPfSUB1.
Using a consensus sequence derived from these results, a databank search revealed that many peptides isolated from skin secretions of different frogs would be potential substrates for such an enzyme.
It was suggested that further, nonradical steps catalyzed by MoaA would lead to a PMP-triphosphate intermediate that would be the substrate for MoaC.
Therefore, isomerization reactions at equilibrium (e.g. the inter-conversion of citrate and cis aconitate in the TCA cycle) would not be identified, even though they would be considered substrate cycles by our definition.
It has been suggested that, since antisense ncRNAs are perfectly complementary to their cognate mRNA, the two species could form double stranded RNA (dsRNA) that would be a substrate for the RNA interference system (RNAi).
Mutated lysosomal enzymes are the underlying cause of a number of lysosomal storage diseases involving the accumulation of materials that would be the substrate for the corresponding hydrolases, were they not defective.
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