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5) It would be informative to explain in the paper that the 25 somite stage is ∼when the tailbud forms and that this is when when cell ingression through the primitive streak ceases refer to work from Susan Mackem's lab in chick showing cessation at HH stage16 (∼ 24-28 somites, Knezevic et al, 1998 and see Catala et al, 1995, and also Wilson and Beddington, 1996, in the mouse).
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In order to better understand this process, it would be informative to investigate the muscle characteristics in those stages; myoatrophy, for example, may explain this change in the response.
3) As ZBTB16 knockout mice are viable, it would be informative to provide or discuss any phenotypes of these mice that can be explained by a defect in autophagy.
Further information on the intermediate metazoan genes would be informative to address this intriguing issue.
Further studies with larger sample sizes would be informative to strengthen our conclusion.
While either outcome would be interesting, it would be informative to address this issue.
We agree it would be informative to compare the two models.
It would be informative to see how these CNAs co-segregate.
It therefore would be informative to have patient-derived astrocytes included in these disease models.
It would be informative to understand the underlining basis of the discordant calls.
It would also be informative to study these diel transcription patterns under nutrient limited conditions.
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