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We show both the performance of the best and the worst reference selection, which indicates the performance limits of the REFB estimators.
The mean fitness has a magnitude comparable to that of the two worst reference setup runs.
Comparisons of the different methods of candidate reference gene selection tend to identify the best and the worst reference genes consistently [ 24- 26] when evaluating reference genes whose functions are well defined.
According to the GeNorm tool, the TUBA was by far the least reliable reference gene when all samples were combined as one set, whereas for barley and oats the worst reference gene was EIF4A, and for wheat it was TUBA.
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In Fig. 1, the location error for node 5 is probably greater than the location error for node 7. The algorithm attempt to mitigate propagated errors by ignoring the worst references.
For comparison, we considered as a worst case reference the result when CoM2 is applied to the data, simply ignoring that the ICA assumptions are violated at some sample times.
This is the reason why the best and worst performing reference panels were different according to the genotype panels used.
As seen on this latter, patients with low expression (below the median) of both FGFR2 and FGF4 have the worst prognosis (reference group).
As seen on Figure 7.d., in our lung cancer study of early lung adenocarcinomas treated by surgery alone, patients with low expression (below the median) of both genes MCL1 and BCL2 have the worst prognosis (reference group).
Recent atypical events evidence that consequences may exceed by far those of worst-case reference scenarios.
At worst, they produce references to a "crusade" against those jihadist enemies.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com