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We also thank the Caenorhabditis Genetics Center for providing worm strains.
We thank the CGC for certain worm strains and the UNC Psychoactive Drug Screening Program (Bryan Roth and XP Huang) for receptor binding assays.
This article presents points to consider when choosing a microscope, designing an imaging experiment, or selecting appropriate worm strains for imaging.
We thank Cochella lab members for various fosmid reporters; F. Schnorrer (IBDM, Marseille, France) for muscle-specific Gal4 driver lines; and the CGC (NIH-P40-OD010440) for worm strains.
"We agree there is a glitch in one of the worm strains used in the 2001 paper," Dr. Guarente said in an interview.
The Toolkit consists of two components: 1) a series of vectors for DNA assembly by homologous recombination (HR) in the yeast, Saccharomyces cerevisiae, and 2) a set of ten worm strains that each express a single, spectrally distinct fluorescent protein, under control of either the daf-21 or eft-3 promoters.
We also thank J. Ahringer and J. Audhya for worm strains and C. Cowan and M. Gotta for feeding clones.
The worm strains were grown at 20°C on NGM agar plates seeded with E. coli OP50 [41].
We thank the Caenorhabditis Genetics Center, the National Bioresource Project for the Nematode and the Guarente lab for providing worm strains.
The following worm strains were used: wild type (N2, Bristol) and NM1968 slo-1 js379) slo-1 js379ferred worms to assay plates as follows.
The authors are grateful to the Caenorhabditis Genetics Center and Paul Sternberg for providing worm strains and Laura Piddock for providing bacterial isolates.
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