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The authorities identified the author of the latest worm strain as Dan Dumitru Ciobanu from the northern Romanian city of Iasi.
The mutant worm strain was named shg372: c30f12.4 (ushg372.
In the worm strain that stayed on the lawn, they spotted mutations in a gene called HECW1, which is involved in relaying signals through the nervous system.
The worm strain PD4251 was provided by Andrew Fire.
We accounted for background worm strain lethality, defining a worm strain adjusted lethality (L) by the equation L = (1 − W * R, where R is the raw lethality resulting from a given dsRNA fed to that worm strain.
Subsequent OLR revealed a significant effect from the factor worm strain or worm genotype, and also the interaction between the bacterial strain and either worm strain or genotype.
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"We agree there is a glitch in one of the worm strains used in the 2001 paper," Dr. Guarente said in an interview.
This article presents points to consider when choosing a microscope, designing an imaging experiment, or selecting appropriate worm strains for imaging.
The worm strains were grown at 20°C on NGM agar plates seeded with E. coli OP50 [41].
We thank L. Avery, C. Bargmann, O. Hobert, R. Horvitz, M. Nonet, W. Schafer, and the Caenorhabditis Genetics Center for worm strains.
The authors are grateful to the Caenorhabditis Genetics Center and Paul Sternberg for providing worm strains and Laura Piddock for providing bacterial isolates.
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