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Thus is it vital that any method to inhibit worm movement does not subsequently alter regeneration.
These differences may be due to the fact that the mammalian system uses purely cholinergic connections at the neuromuscular junction while worm movement is modulated by both GABAergic and cholinergic synapses.
Worm movement was recorded for 30 min at three frames per second, and the first 2 min are ignored.
Worm movement was measured by digital calibration of distance moved per second during bursts of forward movement.
Camera analysis of worm movement showed a reduced motility in parasites incubated with bleach, compared to media only controls (p < 0.001 for all).
An Andor camera was employed to video parasite movement over a 10 20 s period, and Matlab software was used to calculate the change in pixels resulting from worm movement.
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Worm movements could be studied for several hours.
Conversely, when the current speaker corresponded to a candidate not favoured by a worm, movements in the positive direction were 25% less steep than the unbiased worm, and movements in the negative direction were 25% steeper.
The stimulatory response of acetylcholine was blocked by aqueous extract on whole worm movements.
The path was then smoothed using a moving average filter, and potential short gaps in the position data were identified, which may reflect the neuron temporarily leaving the field of view due to unexpected worm movements during the recordings.
Single worm movements within a liquid system were recorded using a digital CCD camera (Moticam 2300, Motic, St. Ingbert, Germany) coupled to a microscope (SMZ 168, Motic, St. Ingbert, Germany) with a subsequent analysis using the program DanioTrack (Loligo Systems, Tjele, Denmark).
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