Sentence examples for worm line from inspiring English sources

To further investigate whether hαSyn expression affects dopamine homeostasis in worms, we crossed our hαSyn expressing line with a worm line expressing CAT-1 GFP CAT-1 GFP

The less severe cytotoxicity of hαSyn in their worm line, compared with our hαSyn expressing lines and a line reported by Caldwell's group [22], may be due to different levels of protein expression.

The data presented for each worm line are the mean of the three replicates.

A worm line transgenic for hus-1 gfp, hus-1 gfps-1(op241) I; unc-119(ed3)III; opIs34, WS1433ed for detecting thus-1 op241ge hus-1 op241ls.

To clone col-99 cDNA, two μl of RT-reaction products from the worm line col-99::egfp::flag (for details of the worm line, see the following section of the Methods) RNA extract were used as a template in a PCR using a Q5 High-Fidelity DNA Polymerase kit (New England BioLabs) according to the manufacture's instruction.

All the PCR primer sequences for col-99f cDNA and human collagen XIII cDNA with EGFP tag cloning, transgenic worm line verification, and RT-PCR are provided in the table.

In this chapter, we discuss methods for the expression and imaging of fluorescent proteins in the C. elegans germline, including the design of transgenes for optimal expression, the generation of transgenic worm lines by ballistic bombardment, the construction of multimarker lines by mating, and methods for live imaging of the gonad and early embryo.

Interestingly, hαSyn expression did not cause degeneration of DAergic somas in their worm lines either.

One of the resulting worm lines (VF11.1) was crossed into hmt-1 gk161 hmt-1 gk161erate the hmt-1 mutant strain (VF12) expressing HMT-1::GFP under the control of hmt-1 promoter (hmt-1(gk161)III;gfIs1[7hmt-1-hmt-1::GFP,unc-119+]).

The genomic region encompassing the cat-2 genetic lesion (stop codon) was sequenced from worm lines where 100% of the F3 generation expressed GFP (sequencing repeated three additional times).

For each construct, two or three transgenic worm lines were generated.