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Repeatability (intra-day) and intermediate precision (inter-day) were assessed by analyzing working standard solutions.
Consequently, the measurement uncertainty associated with the calibration of working standard microphones, multifunction acoustic calibrator and A-weighted sound pressure level measurements is also reduced.
Each working standard was spotted three times on TLC plate.
See an example of the working standard dilution scheme in Table 1 below.
The chromatogram of working standard of GLM solution was shown in Figure 2.
Each working standard was spotted in triplicate, total 18 tracks were spotted on each TLC plate.
Repeated analyses of the working standard (Merck cellulose) showed that measurement uncertainties are less than 0.3‰.
Both the stock and working standard solutions were stored in a refrigerator.
The stock solution was used to prepare the working standard solution of moxifloxacin and prednisolone.
The working standard solutions of the respective drugs were prepared by serial dilution using water.
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(e) Working standards: Prepare Working Standard Solutions from the chosen stock standard.
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