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We calculate working concentrations of metabolite of this concentrated stock solution.
The working concentrations range from 0 to 400 ng/mL.
The working concentrations were prepared by approximate dilution of standard drug solution.
The stock solution was further diluted appropriately to get working concentrations.
Catalyst systems employing higher temperatures and greater amounts of catalyst could be used to achieve higher maximum working concentrations.
AuNP working concentrations were prepared from the 1,000 μg/ml stock preparations in EMEM/S + or EMEM/S-.
The working concentrations were 0, 4, 12 and 20 μM. Figure 7 showed that hemagglutinating activity of RBUP at 2.0 mg/mL was 32 U.
The working concentrations were 0, 4, 12 and 20 μM Fig. 6 The effect of RBUP on A549 cells as observed by using FITC/PI staining.
Immunostaining was performed as previously described using the following primary antibodies and working concentrations.
Stock SNO-cys was diluted to working concentrations in actin buffer <30 seconds before use.
It was then diluted into various working concentrations right before use.
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