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The apoptotic properties of the AuNPs were tested with comet assays on eukaryotic cells to estimate a workable concentration for therapeutic use with minimal side effects.
As shown in Figure 1c, sorafenib could evidently abrogate TGF- β-mediated phosphorylation of Smad2 and Smad3 at a workable concentration of 5 μM.
Using the lowest workable concentration also enabled us to minimise the amount of DMSO added with the Laurdan, reducing any physiological effects of this solvent on the cells.
The workable concentration range for the method was 1 2500 nmol l−1 with inter-batch precision and accuracy in the same concentration interval being 6.1 and 102.9%, respectively.
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The analysis shows that the lower limit of workable ammonia concentration decreases with increasing turbine inlet pressure.
Therefore, we investigated the hypothesis whether co-treatment with a chemotherapeutic drug that functions by interrupting DNA synthesis in the S phase of the cell cycle would enhance the inhibitory effect of SST on gallbladder cancer cell growth and thus lowers the workable SST concentration.
The overnight culture (S. aureus in BHI media) was diluted in BHI medium to a workable optical density (0.5 OD at 600 nm) for cell-counting, then to a working concentration of 10 cell forming units (CFU /200 μL.
However, the experimental estradiol-loaded microemulsion with higher permeation rate was expected to provide effective therapeutic concentration in a workable administration area.
Just before doing the lab, check the indicator and dilute the concentration so that a workable number of drops of fresh orange juice (5 to 25) turn the indicator pink.
Is "liquid democracy" workable?
And are they workable?
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