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Alternatively, ERFAD and ERp90 could work on substrates already bound by SEL1L or OS-9, and potentially also assist the transfer of substrates from either of these proteins to a retrotranslocation channel.
For example, isolated embryonic cardiomyocytes are shown to overstrain and stop beating on rigid substrates, beat but do little work on soft materials, and optimally striate and transmit contractile work on substrates that match the stiffness of their native matrices.
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Enzymes are bio-chemical substances which selectively work on substrate.
These studies imply further work on substrate-competing inhibitors of the JmjC KDMs will be worthwhile.
Déziel and Malenfant reported peptidic substrate-based β-lactams built both from their previous work on substrate-based inhibitors described above and on the β-lactam scaffold.
Although these two proteins work on similar substrates, ALKBH2 prefers double-stranded substrates, whereas ALKBH3 favors single-stranded nucleic acid substrates.
dbPTM is an important resource for researchers working on substrate specificity of PTM sites [14].
The first hypothesis for gene clustering fits well with acquisitive evolution at the origin of metabolic pathways: enzymes in the pathway may derive from related polypeptides because they work on related substrates (see Danchin, 1989 for a discussion).
Traditional SVM algorithms, such as used by recent related work on protease substrate prediction [32], [38] require selecting a single kernel function and using it on all input data throughout the algorithm.
We applied PCA to obtain a reduced set of traction force patterns which the cell used to exert mechanical work on the substrate.
This observation implies that crude extracts may have different effects on stability/activity of the fungal xylanases accumulated in chloroplasts, while SDS-PAGE conditions could provide physical segregation from probable inhibiting and/or degrading agents present in crude plant extracts, allowing the separated enzymes to "work" on their substrate in the "protected environment" of the gel matrix.
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