A fourth limitation relates to the inclusion of the multi-functional anti-inflammatory mediator TGF-β1 in our model, although our earlier work on assaying biochemical markers of vocal fold wound healing failed to detect this mediator in laryngeal secretions pre- or post-traumatically [48].
Todd landed a position as a research scientist at Rigel Pharmaceuticals, a biotechnology company with 120 employees, where he worked on assay development for drug targets and did experimental biochemistry.
In this work, a rapid fluorescence "switch-on" assay was developed to detect trace amount of GSH based on carbon dots MnO2 nanocomposites, which was fabricated through in situ synthesis of MnO2 nanosheets in carbon dots colloid solution.
SSK co-worked on antioxidant assays and analyzed the data for antioxidant assays.
We want to thank Margo van Schie-Troost and Marja Kersbergen-van Oostrom for their work on the cytokine assays, and Peter de Knijff and Dennis Kremer for assistance in the genetic studies.
The authors wish to thank Tessa Moccatta for her assistance with measuring protein carbonyls, Tania Kamal for her work on the ELISA assays and Hong Liu for his assistance with gene expression analysis.
SKP, SK, and DS conceived the project and wrote the manuscript; SK and DS performed the experimental works on MTT assays, RT-PCR, FACS, and chromatin condensation in the two different cell lines; MD carried out the immunocytochemistry and immunohistochemistry; AS and SP participated in the data representation; SR and NP performed the statistical analysis.
We thank Meng Xu and Ricky Alexander for their work on developing the meconium assay.
We would like to thank Ulrike Leurs for her initial work on the iMALDI PRA assay, and Norine Freedman, Lynn Coleman, and Karen Green for performing the PRA analysis by RIA.
The trypan blue and LDH assays work on the same principal, porous membranes allowing the passing of molecules.
We successfully adapted our assay to work on rice.
