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Subjects returned as inpatients for the final 2 wks of intervention, during which the procedures were repeated while the subjects consumed their assigned glucose- or fructose-sweetened beverages as part of an energy-balanced diet.
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At baseline, the low-dose group had a mean plasma ascorbate concentration of 23 μmol/L and this increased to 46 μmol/L after 6 wk of intervention.
Plasma ascorbate concentrations of the 2 groups were statistically different from each other within the first week of intervention, and this difference was maintained for the entire 6 wk of intervention (P < 0.001).
After 6 wk of intervention, there was a ∼3.5-fold increase in mean muscle tissue ascorbate concentrations to 53 and 61 nmol/g tissue in the low- and high-dose groups, respectively (P < 0.0001; Table 3).
After 6 wk of intervention, the urinary excretion of ascorbate had increased 2-fold in the low kiwifruit-dose group and 15-folow kiwifruit-dosee group (Tande 3 ), which suggested that the higher intake of kiwifruit resulted in plasma concentrations close to saturation (18).
After 6 wk of intervention, mononuclear cell ascorbate concentrations increased ∼1.5-fold for both the low kiwifruit-dose group (P = 0.004) and the high–kiwifruit-dose group (P = 0.004), whereas neutrophilow kiwifruit-dosetrations increased >2-fold (low-dose group: P = 0.0042; high-dose group: P = 0.003; Tande 3).
After 12 wk of diet intervention, mice in O-mock group revealed mild but significant LV systolic dysfunction (depressed LVEF and LVFS on M-mode images).
Liver TG pool size was variable but similarly increased in both CDD and MCDD mice compared with controls after 2 wk of dietary intervention (Table 2).
In an additional study (n = 10/group), livers were fixed in 10% formalin for immunohistochemical analysis after 2 wk of dietary interventions, as described above.
Finally, the third biopsy was taken in the morning corresponding to the first biopsy and within 48 72 hrs after completion of the last training session of the 10 wks interventions of the MYA.
These studies have been limited to 4 wk of treatment.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com