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Thus, for every one fragment sequenced per transcript by EXPRSS there would be 10 ~ 150 bp fragments from RNA-seq, one could reasonably assume that 2 million reads from EXPRSS are equivalent to ~20 million reads of RNA-seq data, without transcript length and reverse transcription biases.
"Yes, she said, not quoting verbatim without transcript, "he's going to talk about personal responsibility, like cleaning up those vacant lots in your own neighborhood".
The total mRNA sequence length in RefSeq was 64.2 megabases (without transcript variants); 14.4%, or 9.2 Mb was in Alu containing mRNAs.
Most interesting from the perspective of gene discovery are the 13,427 unitags without transcript match, but with a single match in the genome (Table 3).
The genes with and without transcript accumulation polymorphism had similar percentage of SNPs, suggesting no or little correlation between transcript SNPs and transcript accumulation polymorphism.
For MEG families without transcript evidence in S. haematobium or S. japonicum, a BLASTn search of S. mansoni transcript sequences against the S. haematobium or S. japonicum genome sequences was performed and alignments with an e value lower than e-5 were interpreted as the presence of an orthologous exon.
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Without transcripts or exhibits, it's impossible to know how Donovan made his case before the grand jury, and to counter mounting public skepticism about the proceedings.
Some genes without transcript-protein concordance were identified, which may arise from annotation errors or differential regulation of translation, turnover or alternative splicing.
In addition, we have technically implemented an artificial 300-bp UTRs for predicted genes without transcript-based evidence and only extracted the 3' most (canonical position) tags from virtual transcripts.
Alternative RNA transcripts from exon 1 or exon 2 with or without transcripts of exon 5 lead to several related IGF-1 proteins.
Because animal miRNAs are generally believed to block translational processes without affecting transcript levels, the results strongly indicated the involvement of a miRNA-mediated post-transcriptional regulatory mechanism in CDK6 repression.
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