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Appropriate negative controls without the primary antibodies were performed.
Background control (Bkg) sections were prepared without the primary antibodies.
A negative control was performed without the primary antibody.
Stainings without the primary antibodies were performed as controls.
Negative control sections were used without the primary antibody.
Control staining was performed without the primary antibodies.
As negative controls, some sections were processed as described above, but without the primary antibody.
Stains without the primary antibody, or with control IgG, were used as negative controls.
Antibody specificity was confirmed by parallel stainings without the primary antibody.
As a negative control specimens were incubated without the primary antibody.
To control for non-specific staining, adjacent sections were stained as above, without the primary antibody.
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