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Pre-coated BSA corona decreased cellular uptake of GONs in A549 cells in the media, with and without supplemented with 10% fetal bovine serum (FBS) but drastically increased cellular uptake on HEK 293 cells.
Remarkably, biofilm was formed when bacteria were grown at a starting pH of 6.4, even without supplemented glucose.
Therefore, the BRCA1 antisense subclones were also studied for anchorage independent growth in a semisoft agar medium with and without supplemented estrogen.
All SSF experiments were conducted in the same bioreactors fitted with Rushton impellers and chemically defined medium as described above without supplemented carbon, implying that all carbon supplied to the culture was liberated by enzymatic hydrolysis from the steam-pretreated sweet sorghum bagasse.
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A control experiment was prepared without supplementing the milk base.
The mESC maintenance medium was used for spontaneous differentiation without supplementing LIF, the EsK3β inhibitor or the MEK inhibitor.
CelA2 and BSLA production was performed in TBkan- or LBamp-medium without supplementing IPTG or the induction components glucose and lactose.
These results demonstrated that RGD and BMP-2 mimetic peptides act synergistically to enhance hBMSCs osteogenesis without supplementing the media with osteogenic factors.
But without supplementing the numbers with context, leaders are just guessing about the future of their company.
We further showed that hFLSCs over-expressing bFGF could be used as a new feeder cells to maintain H9 hES cells without supplementing with any exogenous growth factors.
We also found that the conditioned medium of bFGF-hFLSCs could maintain the H9 hES cells under feeder-free conditions without supplementing with bFGF.
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