For example, in the stem cell in vitro experiments described here, one could add any number of additional markers to the E-SLAM protocol and, without changing the sorted cell population, be able to simultaneously assess the new marker's impact on cell proliferation, division kinetics, and differentiation capacities.
To evaluate whether microfluidic sperm sorters (MFSSs) allow effective recovery of sorted motile sperm without DNA damage compared with the centrifugation and swim-up procedure.
As a control, a mixed cell population consisting of GFP+ and GFP- cells was obtained from sorted transfected cultures without selection (GFP+/-), whereas non-transfected cells (NT) (at 3 days in vitro) were used to establish the basal levels of mRNA expression.
For pigmentation sorts, cells were sorted based on forward and side scatter without the use of surface markers.
To further evaluate whether ENG overexpression in TIE2+/CD117+ cells enriched numbers of functional hemogenic progenitors, we performed LDAs with sorted TIE2+/CD117+ cells with or without Dox.
Cell cycle and proliferation curves of sorted populations were similar with and without RO4929097 treatment in both DT-22 and MDA-MB-231 (Fig 6F and Supporting Information Fig S6H J).
Novel linear Ig heavy- and light-chain gene expression cassettes were designed to express Ig VH and VL genes isolated from sorted single B cells as IgG1 antibody without a cloning step.
Without telling anyone, Kiefer began collecting the items, stashing them in sorted piles in the garages of friends.
Some scientists are concerned with taxonomy, sorting like with like without regard to how the sorted items came about or how they work.
Purity of sorted blood cells was ≥97.8% as determined by flow cytometry (without debris).
Furthermore, our definition of NKT cells appears valid, since TCR sequencing of sorted cord blood NKT confirmed the typical invariant Vα24-Jα18 Vα24-Jα18 wiTCRut N-additions.
