For predicted genes without sequence similarity to known genes, only those equal to or longer than 150 bp were considered as candidates.
He "wrote as he thought, and as all men think, without sequence, without an eye to what went before or should come after," said his close friend and his greatest critic, William Dean Howells.
Without sequence information, scientists have one other clue to go on: the first Korean patient.
Results of this work demonstrate the potential of microarray technique to detect viral pathogens without sequence bias amplification of template RNA.
Duplex RT-PCR using the newly designed primers successfully detected all VHSV isolates and validated the genotype (I and IV) without sequence analysis.
"Many useful genes are carried in traditional [rice] landraces; without sequence information it is difficult to use such treasure," he says.
Therefore, it is undoubtedly that MrkH binds to DNA without sequence specificity in vitro, and thus binds to longer DNA strand more tightly.
For PNA targeting gene, peptide-PNAs (PPNA) activity cannot be occurred without sequence homology, at the same time, it has been affected by sequence-based specific target and dose-dependent-based manner.
The structure consists of a DNA-based biomaterial layer sandwiched between two electrodes, where the DNA-based biomaterial is solution-processed without sequence control or external doping of nanoparticles.
The value of recognising potyvirus species without sequence data is uncertain.
Without sequence diversity in probe regions we would expect as many cisB6 eQTLs as cisD2 eQTLs, that is, 30.
