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In a six-well plate, 1,000,000 cells from the selected sublines were seeded (without selection chemicals).
I'd like that for everyone, without selection.
This resistance was unstable after five generations without selection.
Transformation to generate the PpHTB2-mRFP lines was performed without selection marker insertion (Supplementary Fig. 5a).
Those are really tough areas in London where they're actually achieving higher educational standards than in Kent and without selection".
Using optimized conditions, point mutations can be made with such high frequencies that they can be found without selection.
This technique allowed recovery of marker-less triple-engineering events with 64% efficiency without selection for expression of all gRNAs.
Even the best device without selection is useless".
Cells were harvested 5 days after transfection without selection.
The network model without selection is implemented, for instance, in the neuron diagrams popularized by Lewis.
After entering the GD Community, PA was a free rider that absorbed resources without selection.
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