Exact(7)
The engineered receptor tropisms were stably maintained during multiple serial virus passage without reversion to native receptor usage, even on cells offering the choice of both native and alternative receptors.
Indeed, once transmitted, a drug-resistant virus can persist for months to years without reversion to wild-type [ 5].
However, mutations may arise that compensate for the fitness cost, restoring the bacteria's fitness without reversion of the resistant phenotype.
The progression of albuminuria was defined as an increase of albuminuria for at least two successive yearly visits without reversion to an improved level.
Infections of complementing (A1-L4) anon-complementinging cells (293TETOFF) were compared to confirm that the intended virus had been rescued without reversion.
Worsening albuminuria was defined as an increase in the category of albuminuria at follow-up visits without reversion to an improved level (e.g., from no albuminuria to either micro- or macroalbuminuria or from microalbuminuria to macroalbuminuria).
Similar(53)
Our analysis of the virus genomes at early stages of propagation revealed a large collection of individual clones with and without reversions.
This analysis suggested that the streptophyte algae regularly (without reversions) evolved towards increasing morphological complexity (resulting in a larger number of shared morphological characters with embryophytes).
Another limitation of our work is that we only examined two evolutionary trajectories (the main trajectory and the trajectory without reversions).
Therefore, we have added the following to the Discussion: "Another important limitation of our work is that we only examined two evolutionary trajectories (the main trajectory and the trajectory without reversions).
This failure to reach wild-type activity levels without reversions, as well as the fact that three out of the five new mutations obtained (p135S, a203E, s308C, Figure 3B) were identical to the successful trajectory containing reversions, emphasizes that the number of adaptive trajectories that lead to a wild-type level fitness peak from AE are highly limited.
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