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Property (b) stems from the fact that without reads, byzantine transactions are executed in a single step.
To compare the relative transcription levels of the four replicons, the mapped read numbers (without reads for rRNA and tRNA genes) were normalized for the size of each replicon.
A fourth possibility, a background derived from sequencing of contaminating DNA, may be discarded, since there are many intergenic regions from which there were no reads (see gaps without reads on Figure 1A).
Given the high sensitivity of the RT-PCR-seq method, 14 MbIGYPs without reads spanning the exon-exon junctions and 2 MbIGYPs with only one read spanning these junctions (Additional file 3) were likely to be inactive pseudogenes.
To control this possibility, we decided to test if the genomic DNA segments without reads mapping on them are also present in the genome of the strain from where the RNA comes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com