Sentence examples for without primer from inspiring English sources

Binding studies of fluorescein-labeled truncated (without primer binding region) D1 and D2 and full length D1 anti-digoxin aptamers were performed and their corresponding dissociation constants values were 8.2 × 10−9, 44.0 × 10−9 and 17.8 × 10−9 M, respectively.

Adhesive A without Primer: A. Adhesive B without Primer: B. Adhesive B with Primer P: BP.

The values are approximately three times to those without primer treatment.

Adhesive C without Primer: C   5. Adhesive C with Primer P: CP  .

Adhesive C without Primer: C. Adhesive C with Primer P: CP.

In this paper, the combinations of adhesives and primer are abbreviated and denoted as follows: 1. Adhesive A without Primer: A   2. Adhesive B without Primer: B   3. Adhesive B with Primer P: BP   4.

The first PCR step is conducted without primers in order to reassemble the DNase I-digested fragments into longer pieces.

The two flanking regions were then fused by PCR using primers NWMN_1067_−998_KpnI_F2 and NWMN_1067_ +1425_SacI_R2 by combining equimolar ratios of the flanking regions and cycling without primers for five cycles followed by another 30 cycles after addition of the primers.

The DNA pellet was dissolved in 50 µl standard PCR reaction mixture (components see above, without primers) containing 1 unit Taq DNA polymerase.

A further control was the use, on the same slide as the test section, of a duplicate section to which PCR mix was added without Taq and another section added without primers.

Using the primers CAP-F (5'-CGTGTATGCAAGTACATTAC-3') and CAP-R (5'-CTGATTAGTCATTAGTCCATC-3'), we amplified a fragment of 598 bp (without primers) that corresponds to the positions 15,653 to 16,250 on the complete goat mitochondrial sequence of reference ([42]; accession number AF533441).