Sentence examples for without mismatches from inspiring English sources
Reads mapping to the respective genomes without mismatches were then used to obtain expression profiles for the miRNAs.
From the collection of reads mapping to the genome without mismatches we removed reads mapping to pre-miRNAs, tRNAs, rRNAs, snoRNAs, snRNAs, and piRNAs.
We explicitly tested for the first time the 'environmental specificity' of traditional 16S rRNA-targeted Fluorescence In Situ Hybridization (FISH) through comparison of the bacterial diversity actually targeted in the environment with the diversity that should be exactly targeted (i.e. without mismatches) according to in silico analysis.
These larger sets include also lower affinity sites and accordingly display a lower frequency of 'perfect' GAS without mismatches.
It is easy to see that short reads that do not map perfectly (i.e.: without mismatches) to any known regions of the genome are by definition absent words in the context of the genome sequence.
The probe percent bound value, calculated using multi-state equilibrium models of solution hybridization, is shown to be useful in understanding the hybridization behavior of microarray probes having 50 nucleotides, with and without mismatches.
Qin et al. compared microarrays to RT-PCR using mouse heart tissue and Pearson correlation of fold change as a performance metric, and found that MAS5, dChip-with-mismatch, and GCRMA outperformed dChip-without-mismatch (analogous to our "MBEI"), RMA, and VSN [7].
In addition, this control method is effective for the drive system with and without mismatched terms.
Genomic coordinates of reads mapping to the genome without mismatch were obtained (in BED format) using a Python script from mapping results.
This approach has shown to be efficient and to allow for local perturbations, making possible the match of individual wells without mismatching others.
Of these, we excluded 3734 from further analyses because they were not significantly expressed above background with respect to at least 10% of all samples (excluding GSE51440 samples generated from HT HG-U133+ PM arrays without mismatch probes).
