Exact(41)
Thereafter, capital ships were built without medium guns.
On day 3, 5 μmol/L Wnt inhibitor IWP4 (Stemgent) was added into RPMI/B27-insulin and cells were cultured without medium change for 48 h.
The plates were infected by spinfection of the cells at 1850 r/min for 1 h at room temperature in the presence of polybrene (4 μg/mL) and put back in the incubator without medium change.
The acute toxicity (96 h) of pyrene (PY) to European seabass (Dicentrachus labrax) juveniles assessed in a semi-static bioassay (SSB) with medium renewal at each 12 h, and in a static bioassay (SB) without medium renewal was compared in laboratorial conditions (water PY concentrations: 0.07 10 mg L−1).
It should be noted that in this case the analysis of MVOCs was carried out for a mould growing on actual historic wool sample without medium, sorption of the microbial volatiles was carried out in a shorter time than for the samples in vials, and additionally, the sorption was performed in the open system.
After incubation for 10 14 days without medium change, cultures were washed with HBSS, fixed with 100% methanol and stained with an aqueous solution of saturated methyl violet.
Similar(19)
Primary chondrocytes were seeded onto these different collagen matrices and cultured with or without differentiation medium.
In addition, we compared the three-phase slurry bed methanol synthesis with and without supercritical medium.
At examinations without contrast medium, the full-dose CT was used for attenuation and scatter correction.
Parasites were kept in culture for 32 h without further medium exchange.
Before start of radiotherapy, a planning CT in 3 mm slices is taken without contrast medium.
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