Yeast colonies resulting from transformation with 'empty' vector without insert produced a band of approximately 6.0 kb (vector pEB upsA-ATS was used as negative control, Figure 2).
In the first and second library segments 6%and1.2%2% clones were observed without insert, corresponding to 97% of insert-containing clones for the entire library.
In some cases, the most effective approach can be leading from behind by observing, listening, and pushing the conversation without inserting a point-of-view.
However, its presence restores a cooperative reversible thermal unfolding, with a simple two-state transition, characteristic of BlaP without insert.
The TcPR-4b gene was cloned in pET28a plasmid and was successfully expressed in Escherichia coli Rosetta while no visible band was observed in the controls (pET28a without insert in presence or not of IPTG).
Vector indicates the use of a yeast two-hybrid plasmid without insert.
For the final analysis, we took into consideration only the insert of MFBs without "cardiomyocytes contamination", lower panel).
The average molecular weight of the inserts and the percentage of vectors without inserts were estimated based on sampling of 82 clones from every 50th plate.
These were spotted at multiple positions in addition to the 1,435 cDNAs, and included controls such as PCR mix (without any insert); controls of the SpotReport™ 10-array validation system (Stratagene), and cDNAs corresponding to constitutively expressed genes such as β-actin, γ-actin, glyceraldehyde-3-phosphate dehydrogenase, human ornithine decarboxylase and cyclophilin.
The helical inserts produced much higher fluxes at low cross-flow rates than membranes without inserts (up to a factor of 6 higher), and also performed better than a plain cylindrical insert which had a similar pressure drop versus flow characteristic.
