Sentence examples for without insert for from inspiring English sources
Two types of Drosophila cells, S2R+ and Kc cells [ 49], were transfected with pGL3-basic vector DNA with insert, or without insert for control experiments in 96-well plates.
The presence of non-isothiocyanate products (organic thiocyante, epithionitrile, simple nitrile) in dichloromethane extracts of the assay mixtures after 30 min incubation is indicated by +, the absence by - (in comparison to assays with extracts of bacteria harbouring the expression vector without cDNA insert, for chromatograms see Additional file 5: Figure S3).
In the first and second library segments 6%and1.2%2% clones were observed without insert, corresponding to 97% of insert-containing clones for the entire library.
Human Cot-1 DNA and plasmid DNA without insert as well as a hybridization mix without DNA served as negative controls for hybridization.
To obtain cells for control experiments HeLa T-Rex cells were transfected with pcDNA4™/TO/myc-His without insert.
Clones without insert or with inserts shorter than 500 bp were not detected.
As control, the pZeoSV vector without insert was used.
The same plasmid without insert was used as negative control.
Retinal microglia were activated by exposure to LPS (1 µg/ml for 6 hrs), washed extensively with media, and then co-cultured with primary RPE cells on an adjoining tissue-culture insert for 24 hrs, without directly exposing target RPE cells to LPS.
