Sentence examples for without insert from inspiring English sources

We found that the transfection experiment displaying least influence on the expression pattern shows the highest viability of cells (FuGENE 6 Transfection Reagent with plasmid without insert).

By transfecting a plasmid without insert, expression levels of 12 genes were affected by both transfection reagents, indicating a specific pattern for the transfection procedure with this plasmid (Fig. 1a). Figure 1: Alterations in gene expression after transfection.

(a,b) The changes in expressed genes were compared between transfections with FuGENE 6 Transfection Reagent and reagent A for the plasmid without insert (a) and the plasmid coding for SEAP (b).

All the FSLW joints (without insert) fractured within top sheet but not along faying surface, suggesting that the shoulder plays an important role comparable or superior to pin in FSLW of thin sheets.

To investigate whether the transfection reagent causes side effects, we compared gene expression (using human GeneChip HG U133 Plus 2.0) after transfection of HEK 293 cells with either the FuGENE 6 Transfection Reagent or reagent A. We analyzed the expression profile of cells transfected with a plasmid without insert (pM1-MT) compared with one coding for the secreted protein SEAP (pM1-SEAP).

Cells transfected with FuGENE 6 Transfection Reagent and the plasmid without insert show dysregulation of only a very small number of genes involved in cell signaling and cell cycle, and no change in the regulation of genes involved in apoptosis, stress response or immune response (Table 1).

Firstly, since it is a feasibility study, only one porcine tibia with a tibial component without insert and femoral component was used, and results were analysed by one imaging specialist.

As a background control, pGL3-TATA without insert was used and treated as described above.

Clones without insert or with inserts shorter than 500 bp were not detected.

To obtain cells for control experiments HeLa T-Rex cells were transfected with pcDNA4™/TO/myc-His without insert.

The RT PCR for human-IL-1β, human-TNF-α and human-IL-6 in HARA-B cells with or without insert-culture with astrocytes was also performed but the fold increase in mRNA was not significant for either cytokine (data not shown).