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Thus, a psychrophilic-based simple biocatalyst can be constructed by using mostly mesophilic enzymes without inactivation by heat treatment.
This may be especially important for MLV-based vectors, which are often used without inactivation of the LTRs, and which may regain replication potential in the presence of a natural human retrovirus[20].
The step current showed rapid activation and outward rectification without inactivation as that described in HEK 293 cells stably expressing TRPM7 gene [ 25].
The deletion of the adaptor also provided an opportunity to incorporate longer DNA fragments between the TATA box and the transcription initiation site without inactivation of URA3.
Based on our determination of pH stability (Fig. 7a), it will be necessary to provide FumI in a formulation that enables shuttling through the stomach without inactivation at low pH.
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Cell extracts were then either heat inactivated (negative controls) or used without heat inactivation.
The best acid concentration for the reaction without enzyme inactivation was determined to be 0.3 M.
The inulin concentration on the chicory roots was determined using HPLC after the drying process with and without enzymatic inactivation.
For spores of both strains on wiring insulation, the conditions without complete inactivation all had two or more predictors at the lowest settings.
For wet spores of both strains, the cases without complete inactivation were at the lowest setting for temperature with only one exception.
Heat-treatment of raw starch at 70 °C for 15 min in the presence of adsorbed cyclodextrin glycosyltransferase allowed the starch liquefaction without enzyme inactivation.
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