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Thus, without further sequence coverage of both the prokaryotic and viral metagenome the implications of these results are as yet, unclear.
First, the exact boundaries of predicted CNVs cannot be precisely determined using microarray-based platforms, and it is therefore impossible to characterise the breakpoint sequences that are involved in the copy number change without further sequence analysis.
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Therefore using GWAS, especially in gene-dense regions, one cannot usually make conclusive judgements about which gene(s) is causal without further sequencing or functional analysis.
In this study, the number of distinct bands from SSCP blots was used to indicate clones, however, the homogeneity of such bands or spectratype peaks [ 6] cannot be assumed without further sequencing.
However, without further sequencing of this genomic region from other cnidarians, it is unclear if the two genes originate from an ancestral ace operon but one of them was inverted in the sea anemone or the current gene organization is an assembly artifact.
Without further genomic sequences testing, we also could not draw conclusions based on the RT-PCR outcomes, because we had not found the same sequence among different samples.
The PCR product sizes of the PM1 alleles were then used to identify and define the alleles in the individual samples without the need for further sequencing.
Sequences without ambiguous base calls yielding a significant hit for Viridiplantae were further sequenced with the reverse primer.
To further demonstrate that the identified base substitution is indeed the mutation causing the kar phenotype, and not simply a polymorphism in the zebrafish genome without further consequence, we sequenced the site in 49 wild-type fish from different backgrounds.
Sanger sequencing of 12 aN bands of sufficient intensity to sequence without further amplification, confirmed the events (data not shown).
The dataset was used without further regard to chimeric sequences because putative chimeric sequences composed a minuscule fraction of reads.
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CEO of Professional Science Editing for Scientists @ prosciediting.com