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All experiments were performed using RA synoviocytes within the fifth passage, and using RA synovium-infiltrating lymphocytes without further passage.
These viruses (without further passage) were diluted in Eagle minimal essential medium (MEM) containing antibiotics and 10% antibody-negative normal horse serum before being used.
Similar(58)
The experiments were carried out from cultures growing from the frozen stock without further passages.
DENV isolates were used for analysis without any further passage.
Without any assistance, the task force got within 8 mile of the Confederate capital but could not proceed further because of sunken vessels and debris placed in the river blocking further passage.
Candide buys their freedom and further passage at steep prices.
For further passaging, trypsinization was performed according to the manufacturer's instructions.
Further passages were performed upon confluence.
For further passages cells were detached by trypsin digestion.
With further passaging, we established multiple Blimp1-null rESC lines (4/10).
In further passages, a drop in stiffness had occurred.
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