The wide avenues, the new buildings, the old gates, temples and palaces are but a very loose grid filled in with dozens of villages-Asian villages, but un-Asian in being clean, without smells, without beggars and even without flies.
Each experiment included an identical, CAFE chamber without flies to determine evaporative losses (typically 10% of ingested volumes), which were subtracted from experimental readings [21].
We also determined the amount of diet that was lost to evaporation by placing a capillary feeder in a chamber without flies and this value was subtracted from the values determined for the feeding experiments.
After 24 hours, the amount of liquid food consumed was measured and was adjusted to take into account evaporation, which was measured using a sucrose-filled micropipette in a 1% agar vial without flies.
A control for evaporation without flies was included each time.
Apple cider vinegar stimulation alone (without flies) was used as a negative control.
