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Stratified cell sheets were engineered from cloned mouse iPS cells-derived K14-positive epithelial cells by air-lifting culture without feeder cells.
Aim was to study the efficacy of the ET and the SCSM without feeder cells to grow primary cultures and to test the effects of donor age, of extracellular matrix proteins (ECMP), and of autogenous serum on cell growth in explant cultures.
CGR8 cells were cultured in plates coated with 0.1% gelatin (Sigma G9391) without feeder layer cells.
But in our culture condition, chicken PGCs can be maintained without feeder layer (a feeder-free) and BRL-conditioned media.
For transduction, undifferentiated mouse ESCs and human iPSCs were incubated with 50 ul supernatant without feeder cells for 12 hours.
ZHBTc4, ZHTc6, and EB5 ES cells were cultured without feeder cells in LIF-supplemented medium as described [19], [66].
Similar(25)
He pointed out that birds are very well adapted to surviving the winter, that only a small percentage eat seed and suet, and that birds have done well for many thousands of years without feeders.
The initial design is the casting part (without feeders) which is placed in a suitable mold box.
Culture conditions include 'minimal stem cell medium' (hESC media without feeder-conditioned medium) and Hs27-conditioned medium, with and without bFGF supplementation.
C5 cells were cultivated with or without feeders, as indicated.
The B10 cell line was cultivated without feeders.
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