Identifying gene regulatory DNA domains with conserved function but without conserved sequence across species is a daunting task, especially when they are located differently in the gene region as noted here between appb and APP.
The sequences without conserved WRKYGQK domain signatures were discarded.
Genes without assigned orthologs were located in genomic regions without conserved synteny compared to the previously published Giardia genomes.
In the current study, the pseudogenes and sequences without conserved domains from P-loop to GLPL were excluded from further analysis.
Thus, they most resemble the short regional centromeres of C. albicans that also have unique DNA sequence without conserved motifs [ 28, 56, 57].
When the full length Lif 3′UTR was compared to a similar-sized human genomic sequence without conserved, genic or repetitive sequences this reduction was 55%, excluding that Lif mRNA destabilization was mediated non-specifically by its long 3′UTR sequence.
Only exons with a unique conserved ortholog but without highly conserved paralogs are kept.
In order to diminish Fc effector functions of 20D5HCmIgG1, a 20D5HCmIgG1 cDNA sequence variant was made designated 20D5HCmIgG1-Q encoding 20D5mIgG1 without a conserved N-glycosylation consensus site.
HIV-1+ individuals who, without therapy, conserve cellular anti-HIV-1 responses, present with high, stable CD4+ T-cell numbers, and control viral replication, facilitate analysis of atypical viro-immunopathology.
Those species without highly conserved glutamate-153 were: the three alveolate species (T. annulata, T. parva, P. primaurelia), the entamoeba E. histolytica, and one plant species (Brassica napus).
