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HBSS++ alone without compounds was run as negative control.
18 h after treating cells with LPS (5 μg/mL) with or without compounds in RAW 264.7 cells, samples were harvested and lysated to immunoblottings with COX-2 and β-actin antibodies.
RAW 264.7 cells were incubated with 5 μg/mL LPS for 18 h with or without compounds and amount of PGE2 in medium was determined using PGE2-specific ELISA assays (***significant as compared to control, *p < 0.05; #significant as compared to LPS group, n = 5.
Remaining solutions were added by using the WellMate from Matrix Technologies Corp. Controls were included with each plate, corresponding to cells treated with DMSO (without compounds) and cells cultured with and without X-gal.
(B ) Representative images of apoptotic cells with and without compounds that block extrusion.
Cells were treated with or without compounds 6 and 8 for 2 h prior to 24 h of H2O2 exposure.
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CEO of Professional Science Editing for Scientists @ prosciediting.com