Separation and detection was performed directly on urine samples without extensive cleanup and without derivatization.
Therefore, we employed a methodology where cell lysis, mRNA purification and cDNA synthesis occurs in a single well, through the sequential addition of the necessary enzymes and buffers, without intervening cleanup steps, and especially without removing the sample from the well.
Without a cleanup, the material would cling to surfaces and contaminate the area for decades.
Tavarez, who was already in Fort Myers, mentioned it to a newscaster, and thus in a roundabout way the team's manager, Terry Francona, came to understand that he'd be starting the Grapefruit League season without his cleanup batter.
The United States Coast Guard allowed a limited number of ships to use the Calcasieu Ship Channel after the wake from a barge carrying gasoline moved through the channel without disturbing cleanup efforts, said spokesman Petty Officer 2nd Class Adam Eggers.
He was already preoccupied with thoughts of Game 5, which Oakland must play without its cleanup hitter, Jermaine Dye, who fractured his left leg when he fouled a ball off it in the third inning today.
The Marlins played their first game without their cleanup hitter, Mike Lowell, who is out for the rest of the regular season after breaking a bone in his hand Saturday when he was hit by a fastball from Montreal's Hector Almonte.
The Yankees prevailed again although they were short-handed, playing without the cleanup hitter Bernie Williams -- who has been with his ailing father in Puerto Rico and may rejoin the team in Boston on Friday -- and catcher Jorge Posada, who missed today's game because of back stiffness but is expected to play against the Red Sox.
The modified QuEChERS method, based on the procedure by the Association of Analytical Communities official method 2007.01 (Koesukwiwat et al. 2014; Mekonen et al. 2014; Neil et al. 2007) but without the cleanup phase was adopted for the extraction of the spiked samples and blank samples of yam.
