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We observed that nearly 45% of the streptavidin mass remained (for ∼44 h) in the networks without being destabilized by the harsh protease.
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It detests being destabilized.
It cannot risk being destabilized further.
Analysis of these variants by screening for enhanced green fluorescent protein reporter expression and by quantitation of minigenomic RNA levels demonstrated that the upper portions of the putative panhandle and 3′ genomic structures can be destabilized without affecting virus replication.
This indicates that the construct is destabilized without TEV protease cleavage.
A possible explanation for the differences between GFP-cODC1-TDegF-RFP and GFP-cODC1-TDegF mutants might lie in changes of target protein levels induced by the GFP-cODC1-TDegF tag at permissive conditions, as we found that the GFP-cODC1-TDegF construct is destabilized without TEV protease cleavage.
However, unlike microarrays, this assay will allows the location of sites of cleavage on an individual transcript, and although it will miss translationally inhibited targets as well as those where the mRNA has been destabilized without cleavage, it will provide superior results for examining miRNA binding sites for this specific mode of action.
The region is destabilized".
Societies will be destabilized.
Nor has the political system been destabilized.
Thirteen young adults were destabilized with platform perturbations, and reached to grasp a nearby handrail to recover balance without stepping.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com