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GeoTagger shows all the places mentioned on the page without any tagging or other wok required by the publisher.
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Briefly, formaldehyde-fixed nuclei were isolated from HeLa transfectants carrying a TFEB-3xFLAG transgene or a control HeLa cell line without any tagged transgene.
India now has "a cocktail of terrorist groups, without any tag," he says.
But according to Wilson John of the Observer Research Foundation, an Indian think-tank, India now has "a cocktail of terrorist groups, without any tag".
Based on this original vector, a series of transient expression vectors with N-/C-terminal tags of HA, cMyc, EGFP or mVenus or without any tags were generated.
It's about war and about veterans and about the universality of veterans and how everybody's got a veteran in their culture, and they're all on both the wrong side and the right side of history in a way, because they're in conflicts which wind up being essentially human without any tag of nationality.
Similarly, Lig A3 and Lig A4 were also purified as GST tagged proteins, whereas Lig A9 and Lig A10 were overexpressed without any tag.
To exclude the possibility that the FLAG-tag affected the results, we obtained NIH3T3 cells expressing Abi-1 or NESH/Abi-3 without any tag.
When expressed in the cytosol without any tags no significant differences in fluorescence intensities between the two Sapphire variants were detectable (data not shown).
A cDNA segment encoding human CENP-H was subcloned in pUCDM vector, without any tag, under the control of the p10 promoter.
HEK293 F cells (1 × 10 cells/mL) were transfected with pCMV His6-aPKC 259 606 and pCMV dPDK-1 (without any tag) using the 293fectin transfection reagent (Life Technologies).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com