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I say that without any mock heroism.
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Control vectors consisted of recombinant adenoviruses containing an expression cassette without any genome (mock adenovirus vector, Ax-mock).
Interphase extract was used without any treatment, mock depleted, Plx1 depleted or Plx1 depleted and supplemented with recombinant Plx1 (6 ng/μl).
Both the control siRNA transfected cells and cells without any transfection (Mock) showed similar levels of p73 and VDR, thus ruling out the possibility of any non-specific effect due to siRNA transfections.
Boeing used the CATIA package to develop the 777, the first aircraft to have been designed completely with computers without a mock-up.
Churchill also realises the old have aspirations, too – at one point they dwell on the joys of flight – and are not without a mocking humour.
Take a look at the meals that you and your family already enjoy, and you'll probably notice that many of them can be made without any meat or with mock meats (which are great transition foods) instead of animal flesh.
To calculate the fold change of GFP expression with DAZL overexpression, the average of GFP/DsRed ratio was normalized with that of embryos injected without any mRNA encoding DAZL (mock control).
For animal experiments, 6 8 weeks old female BALB/c mice (Harlan Laboratories, Indianapolis, IN) were immunized intranasally with 20 µg of M2 VLPs (M2VLP) or M1 only VLPs without M2 (Mock) with a single or two doses at 4 weeks interval.
The other aliquot was incubated without antibodies (mock).
Cells electroporated without mRNA (mock DCs) were used as negative control.
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Justyna Jupowicz-Kozak
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