Without performing any additional washing steps beyond the previously described SLIDE protocol, we demonstrated highly specific capture of GFP using a silver stained gel.
After centrifugation to remove the lysis solution, the cells were resuspended in culture medium and returned to 37°C for cultivation without additional washing.
Without additional washing steps, the biochip was stained for 15 min with the provided streptavidin-horseradish peroxidase conjugate, and thereafter rinsed with 1 mL of assay buffer.
In addition, ERPs were recorded to vibratory stimuli without any additional task.
Interestingly, its battery pack manages without any additional cooling system.
"But without any additional information, without some type of suspicious behavior, they should not go any farther".
The Better Care Fund is merely the same process mandated from government but, crucially, without any additional resources.
The impacts can emerge without any additional climate warming.
We can design the pulses directly without any additional couplings.
Clean chromatograms were obtained without any additional cleanup step.
