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EVs were obtained from supernatants of ASCs after 2 days culture in DMEM with or without additional factors.
Thus our hotspot motif model is robust enough to account for the repositioning of hotspots and the maintenance of hotspot numbers over evolutionary time scales, with or without additional factors such as shifts.
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That would be complicated enough, even without two additional factors.
Instead, the proposed method uses dynamic matrix control (DMC) to design PID controllers for non-square subsystems without using additional factors.
I therefore purposely formulated simple analytical models without incorporating additional factors affecting population dynamics.
The cells were cultured for several months in media containing N2 and B27 supplement without any additional factors for further differentiation.
Furthermore, the digital impression technique and digital workflow are designed as the "digital working model" directly from the intraoral scan, without any additional factors.
Because our experiments were done in growth media containing additional glutamine, as well as additional nonessential amino acids, which could influence the requirement for autophagy, we repeated our experiments in basal media without these additional factors.
Explanatory conditions for discrepancies between direct sequencing of the cDNA and the DNA analyses could be applied without any additional factors only for those cases presenting hemizygous mutations of P53.
To isolate the effect of ethanol in tSSF without potential additional factors associated with higher solids concentration (e.g. higher cell mass, impeded mass transfer), tSSFs with initial Avicel concentrations of 20 g/L were supplemented with ethanol to initial concentrations of 1.07, 16.82, and 32.17 g/L.
Other studies use a combination of those materials, with or without the addition of additional factors such as PDGF or BMP-2.
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CEO of Professional Science Editing for Scientists @ prosciediting.com