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Infiltrations of the bacterial suspension or saline only as control were carried out by injection without a syringe to the abaxial surface of the leaf as described [46].
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Fe2P nanorods can also be prepared without using a syringe pump as shown in the work of Whitmire et al. [10] who developed a single-source organometallic method to control morphology by varying the ratio of tri-n-octylamine (TOA) to oleic acid (OA) in a one-pot reaction.
Six hours later, at 1500 h, up to a maximum of 1 mL blood/rat was collected by venepuncture of the tail vein using a 23-gauge needle (Terumo Europe NV, Belgium) but without use of a syringe.
While it was known to be common practice for women to take various pills and abortifacient remedies in an attempt to restore menstruation, it was considered extremely rare for women to induce an abortion successfully via internal methods, such as with a syringe, without assistance, and without endangering their lives.
Measure and dispense the 2X methylcellulose using a syringe without a needle.
The bacteria were infiltrated into detached potato leaves using a syringe without needle.
Acetosyringone (200 μM final) was added to the bacterial suspension prior tobacco leave infiltration using a syringe without needle.
Agrobacterium strains carrying each pro-vector module were mixed and infiltrated into N. benthamiana using a syringe without a needle as described [ 12].
Leaves of N. benthamiana plants were infiltrated with suspension of agrobacteria (optical absorption of the solution OD600 ~ 0.2) using a syringe without a needle.
Agrobacterium were collected and resuspended in 5% sucrose at a concentration of OD600 = 2.0, followed by infiltration into tobacco leaves using a syringe without the needle.
After the infiltration of the Agrobacterium suspension into the abaxial surfaces of tobacco leaves from a syringe without a needle [ 37], the tobacco plants were maintained in a moist chamber at 26°C for 48 h for GUS activity analysis.
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