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These results validate the suspected flexibility of the central loop, but raise significant doubt about its direct functional role in GS catalysis via conformational switching, including the proposed regulation of GS via ADP-ribosylation within this loop.
Furthermore, a single rigidifying mutation within this loop can significantly improve the enzyme specific activity, as well as the stability to thermal denaturation and aggregation, to give an increased temperature optimum for activity.
Phosphorylation of a site within this loop secures the conformation of the activation and catalytic loops by stabilizing a cluster of positively charged side chains.
This location for D2 adjacent the pABA binding site is consistent with the presence of sulfonamide resistance mutations within this loop region.
In contrast, no electron density is observed for various residues within this loop for either the unbound form of the BoNT/A LC, or when bound to arginine hydroxamate (ArgHX) (residues 245 256), I1 (residues 250 253), or JTH-NB72-39 JTH-NB72-39 JTH-NB72-39uresidues
There is very low amino acid conservation within this loop.
Similar(44)
This sequence is present within the disulfide loop and confirmed that cleavage of mSEB occurred within this conserved loop region and 4(d)).
As long as he sits within the periphery of this loop in his living room, the t-coil receives the transmission.
Two other mutations occurred within HA2, but outside this loop region (N114S and V122A).
Electron density for residues 258 293 is absent, indicating partial disorder of this loop within the crystal structure.
The modern Plasmodium LDHs have a two-residue deletion within this opposing loop (highlighted in cyan in Figure 7C), while the opposing loop is shared with AncLDH* and the Toxoplasma LDHs.
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