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While their cleavage specificity can be altered using several protein engineering and selection strategies, their overall targetability is limited by highly specific indirect recognition of the central four base pairs within their recognition sites.
This suggests that the adhesins may have an intriguingly simple and effective evolutionary strategy to generate diversity selectively within their recognition specificity domains.
It is worthy to note that these methods are based on the Golden Gate procedure, a cloning strategy that makes use of type IIs restriction enzymes which cut sequences adjacent, rather than within their recognition sites, and allows seamless ligation of repetitive sequences in a specific order [ 5, 17].
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The subclades show significant variability within their substrate recognition sites between the clades, suggesting different biochemical functions and providing insights into the evolution of cytochrome P450 'blooms' in response to environmental pressures.
More specifically, there are studies indicating that social concerns (e.g., the individuals' perception of their role within social groups and their recognition of sociocultural forces) can influence the decision to teach evolution or not (Balgopal [2014]; Goldston and Kyzer [2009]; Shanaham and Nieswandt [2011]).
Latin American countries announced recently their recognition of Palestine within the 1967 borders.
Heads of departments should ensure that teaching within their remit carries recognition and consider rewarding outstanding commitment to teaching methods or development.
Traditionally, galectins have been defined as a subfamily of lectins that selectively bind β-galactosides, and all galectins share significant amino acid sequence conservation within their CRD (carbohydrate-recognition domain) [ 1].
Vocal learning in elephants might be used to facilitate vocal recognition within their fission-fusion society by increasing the similarity between related or socially affiliated individuals (males also use vocalizations to negotiate within the social network).
32 In Benin, as in other parts of Africa, to bear children is not only a deeply personal experience that almost all women actively seek, it is a family and social obligation, the primary and sometimes only way women can acquire social status and recognition within their community, and an essential event in assuring continuity of marriage.
Although yeast and animals both belong to the kingdom Opisthokonta, there is ample evidence for diversification of their core promoters: only some motifs such as TATA and INR are well-conserved, pre-initiation complexes assemble much further upstream in yeast than mammals, and sequence differences within their TBPs affect promoter recognition and the binding of other transcription factors [ 5].
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